lunes, 23 de marzo de 2009




- Introduction

horsdst.gif (68406 bytes)Chronic obstructive pulmonary disease (COPD) is an equine lung disease similar to human asthma. The clinical signs of COPD are caused by an allergic response to the particles in hay dust (see Figure 1). It is most often seen in older horses (greater than six years old) that are stabled during the winter months. COPD is rarely seen in warm, dry climates where horses are kept outside all year. Horses with COPD may exhibit clinical signs such as "heaving" to push air out of the lungs towards the end of exhalation, coughing, weight loss, and exercise intolerance. Wheezes may be heard towards the end of exhalation when listening to the airways with a stethoscope. A mucopurulent nasal discharge (composed of mucus and inflammatory cells) may be seen, especially after exercise. The abdominal muscles of COPD-afflicted horses may hypertrophy (enlarge) and form noticeable "heave lines." Heaves does not appear to be breed or gender related. There is evidence, however, that it may be hereditary.






- Etiology of COPD

Hay contains microorganisms such as bacteria and fungi as well as tiny particles of feed grains, plants, feces, dander, and pollen (see the photomicrograph of a clean hay sample in Figure 2). These tiny particles become aerosolized in hayclnhay.gif (39415 bytes) dust and elicit an allergic response when they are inhaled by COPD horses. While it is believed that the hypersensitivity reaction seen in COPD horses is in response to many different allergens, the primary microorganisms involved in the etiology of heaves are Aspergillus fumigatus, Thermoactinomyces vulgaris, and Faenia rectivirgula. Aspergillus fumigatus is a mold that grows on dead and decaying matter such as poorly cured mldhay.gif (47843 bytes)hay. It is thermophilic ("heat-loving") and can thrive in the high temperatures achieved in decomposing vegetation. A. fumigatus forms spores which become airborne and can be inhaled. These spores are antigenic (they are recognized as "foreign" by the immune system and provoke an immune response) and allergenic. Both Thermoactinomyces vulgaris and Faenia rectivirgula are bacteria which produce spores that become airborne and can be inhaled. All three of these species of microorganisms are numerous in moldy hay (Figure 3 is a photomicrograph of moldy hay).




- Pathogenesis of COPD

COPD is a disease that affects the air passages (trachea, bronchi, and bronchioles) through which air flows into the lungs (see Figure 4). The air passages are lined with layerslung1.gif (17677 bytes) of cells which constitute the epithelium. Below the epithelium is a layer of connective tissue called the submucosa. The epithelium and submucosa together are called the mucosa. Smooth muscle surrounds the bronchi and bronchioles all the way to the level of  the alveoli (the air sacs in the lungs where gas exchange takes place). Contraction of the smooth muscle encircling the airways is known as bronchoconstriction or bronchospasm.

The airways are equipped with natural defense mechanisms to eliminate inhaled particles. These mechanisms include coughing, mucus secretion and removal, and bronchoconstriction. Chronic obstructive pulmonary disease is a delayed hypersensitivity reaction to inhaled allergens (materials that provoke allergic reactions). The natural defense mechanisms in the airways of COPD horses are hyperreactive and, therefore, they overreact when foreign particles are inhaled. Inflammation is also one of the defense mechanisms of the airways but in COPD inflammation occurs in excess and its purpose is not clear.



Four to six hours after a COPD horse is exposed to hay dust, the airways become inflamed and massive numbers of neutrophils accumulate within the air passages. Neutrophils are specialized white blood cells that kill bacteria. In COPD, it is still unclear what role the neutrophils play. It is known, however that the substances normally used by neutrophils to kill bacteria are capable of causing some of the changes in the airway epithelium observed in COPD horses.

walthik.gif (23199 bytes)Each time a COPD horse is exposed to hay dust, its airways become acutely inflamed which causes the airways to become edematous (an abnormal accumulation of fluid in intercellular spaces). Repeated episodes of inflammation can cause the airway mucosal cells to proliferate.  Both edema and proliferation of the mucosal cells thicken the airway walls and obstruct normal air flow during breathing (see Figure 5).



gbltcl.gif (35657 bytes)Airway mucus is produced in the trachea and bronchi by goblet cells in the epithelium and submucosal glands (see photomicrograph of goblet cells in Figure 6). Mucus lining the airways is viscous and sticky so that it entraps inhaled particles. The epithelium of the trachea and bronchi is covered with cilia (Figure 7 is a photomicrograph of ciliated epithelium). These tiny hairlike projections on the epithelial surface beat continuously and transport the overlying mucus layer up toward the larynx where the mucus can either be expelled (by coughing) or swallowed. The mucociliary system provides a means by which inhaled foreign particles can be cleared from the airways.ciliaA.gif (29781 bytes) Stimulation of the irritant receptors lying below the airway epithelium promotes mucus secretion so that more mucus is available to transport inhaled allergens out of the airways. Inflammation of the airways stimulates mucus secretion and causes proliferation of mucus producing cells. In COPD horses, excess mucus in the airways plugs the bronchioles (Figure 9 below is a photomicrograph of a mucous plug in the airways).





The smooth muscle that encircles the airways is controlled by the parasympathetic nervous bronch.gif (24060 bytes)system.  Inhaled irritants stimulate the parasympathetic nervous system to release acetylcholine (ACh). The binding of acetylcholine to receptors located on airway smooth muscle cells causes bronchoconstriction (bronchospasm) which prevents irritants from penetrating deeper into the lungs.  When the mucosa is thickened by inflammation, even a little smooth muscle contraction can substantially narrow the airways and make breathing more difficult (this is illustrated in Figure 8). Air flow is also compromised by the increased production of mucus in response to inhaled allergens. Accumulated mucus and cellular debris in the airways further decreases the diameter of the air passages and increases the effort required to breathe. This increased work of breathing is evidencedmucplg.gif (43079 bytes) by the abdominal push ("heaving") seen when COPD-afflicted horses try to force air out through the narrowed airways during exhalation.

Since the air passages of COPD-afflicted horses are obstructed (see Figure 9), oxygen cannot be efficiently delivered to the alveoli. This results in a low partial pressure of oxygen in the arterial blood of COPD horses. Less oxygen is available, therefore, for delivery to the tissues.  Impairment of gas exchange in the lungs of COPD horses prevents these horses from performing well and results in exercise intolerance.




Coughing expels inhaled particles from the airways. Sensory nerve endings called irritant receptors lie below the airway epithelium. The irritant receptors are stimulated whenhrscof.gif (14314 bytes) inhaled particles or accumulated mucus secretions compress the airway epithelium and deform the underlying receptors. In COPD-afflicted horses,  inflammation makes the cough reflex hyperreactive because the epithelium is damaged, irritant receptors become exposed, and the nerves become more sensitive to stimuli (much as an inflamed wound on your finger makes your finger more sensitive to touch). As a result of the hyperreactive cough reflex and mucus accumulation in the airways, COPD horses cough frequently (see Figure 10).





- Diagnosis

Veterinarians usually diagnose chronic obstructive pulmonary disease based on history and clinical signs. Since COPD is an allergic response to particles in hay dust, it should be determined how the horse is being housed and the type of feed it is receiving. Information supplied to the veterinarian by the owner or trainer about the onset and nature of clinical signs such as "heaving," coughing, or mucopurulent nasal discharge is also very useful. In addition, the veterinarian will want to know about any history of exercise intolerance. 

The veterinarian will conduct a complete physical examination and pay particular attention to the lungs. Horses with COPD usually do not have a fever. Abnormal lung sounds, especially wheezing, become more obvious as the disease increases in severity. The veterinarian will look for evidence of a mucopurulent nasal discharge. If there is doubt about the diagnosis, the veterinarian may use endoscopy or bronchoalveolar lavage.bob8.gif (30290 bytes)



Insertion of an endoscope through a nostril of the horse and into the trachea and bronchi allows the veterinarian to directly examine the air passages. The veterinarian will make note of any edema, hyperemia (the "redness" that occurs as more blood is shunted to areas of inflammation), and the presence and color of mucus accumulations. Figure 11 is a videoendoscopic photograph showing large amounts of mucous in the airways of a COPD-afflicted horse.




Bronchoalveolar Lavage

bal0.gif (14099 bytes)Bronchoalveolar lavage is a process whereby a tube is passed through one nostril of the horse into the peripheral airways and then sterile saline is quickly injected and withdrawn from the air passages through the tube. This sample is then analysed microscopically for both the total number of cells present and the number and percentage of each cell type present (i.e. macrophages, lymphocytes, neutrophils, eosinophils, and mast cells). In normal horses, the predominant cells are macrophages and lymphocytes with neutrophils comprising less than five percent of all thebal11.gif (30738 bytes) cells present (see Figure 12). In horses with severe COPD, the percentage of neutrophils in bronchoalveolar lavage (BAL) fluid may be 50-70% (or more) of the total cell count (see Figure 13). However, horses with greater than 20% neutrophils will likely have impaired lung function and may have COPD.

Blood gas analysis can also be performed to assist in the diagnosis of chronic obstructive pulmonary disease. An arterial blood sample taken when the horse has just been exercised will have a lower partial pressure of oxygen than normal. Although horses usually become hypoxemic (low levels of oxygen in the blood) during exercise, the hypoxemia seen in COPD horses is more pronounced.



- Treatment of COPD

In equine COPD, inhalation of airborne allergens leads to airway inflammation which gives rise to bronchospasm.  Treatment therefore involves prevention of exposure to allergens by environmental management, reduction of inflammation by use of corticosteroids, and relief of airway obstruction by use of bronchodilator drugs.  Depending on the severity of the disease, use of the horse, and facilities available, one or all of these treatments may be used for a COPD-affected horse.   There is no cure for COPD and, therefore, treatments need to be continued for life.


Environmental Management

The simplest way to treat a COPD horse is to change the environment so as to minimize exposure to hay dusts. This can easily be accomplished by putting the horse out to pasture. COPD-afflicted horses put out to pasture will go into clinical remission. If a horse must be stabled, then it is necessary to eliminate the use of straw for bedding and hay for feed. Even though the dust levels in the barn may seem insignificant, research has shown that thedust1.gif (29527 bytes) dust levels in the breathing zone (i.e. around the nose) of a horse eating hay can be as much as thirty to forty times higher than in the rest of the stall (see Figure 14). When a horse is eating a low dust feed such as pellets, the dust levels in the breathing zone are equivalent to those in the stall (see Figure 14). An effective management strategy for stabled COPD-afflicted horses, therefore, is to bed them on shavings and feed them a low dust diet. Feeds low in dust include complete pelleted feed, alfalfa cubes, and grass silage (haylage). Horses in adjacent stalls preferably should be kept in the same manner so as to prevent hay dusts from contaminating the stall of the COPD horse. However, if this is not possible, simply changing the management in one stall can dramatically improve lung function in a COPD-affected horse.

Hay should not be stored near the stall of a horse with COPD. Improving the ventilation in the barn can also help to minimize airborne particles. This may be accomplished by merely keeping the windows and doors open whenever possible or by using more sophisticated ventilation systems.

It is very important to realize that very short exposure of a COPD-susceptible horse to hay dusts can initiate inflammation and airway obstruction that can last for days.   In a study by Fairbairn et al., COPD-susceptible horses were fed hay for seven hours and then put into a low dust environment. Three days later they still had inflamed airways . For this reason, COPD-affected horses should not simply be pastured during the day and then stabled and fed hay at night.  This overnight exposure to dusts will be sufficient to maintain their airway obstruction.


Anti-Inflammatory Drug Therapy

In addition to changing the environment of a stabled COPD horse, it may be necessary to administer anti-inflammatory drugs. Corticosteroids are the drugs of choice for relieving inflammation of the airways. Corticosteroids can be administered by mouth, by injection,Aero.gif (31716 bytes) or by inhalation. When they are administered by mouth or by injection, therapy usually begins with a high dose and, as the horse improves, the dose is reduced to a maintenance level. Inhaled steroids offer the advantage of a high dose within the airways and minimal systemic side effects but a special mask is necessary for administration (see Figure 15). A chart outlining some of the more common corticosteroids used for COPD follows:





Prednisolone Corticosteroid Oral or IM Once daily; may administer every other day if clinical signs are controlled after a course of treatment Few
Triamcinolone Corticosteroid IM 1 dose every 3 months Laminitis
Dexamethasone Corticosteroid Oral, IV or IM Once daily for 2 days, then every other day Laminitis
Beclomethasone diproprionate Corticosteroid Aerosol Twice daily; after 2 weeks once daily Few




Bronchodilator Drug Therapy

Bronchodilators relax airway smooth muscle and relieve airway obstruction. In mildly affected horses, they may be the first line of therapy. They can also be safely combined with anti-inflammatory drugs for treatment of more severely affected horses.  This combination is beneficial because anti-inflammatory drugs can reduce airway wall thickening but have no direct effect on the smooth muscle regulating the diameter of the airways. Bronchodilator drugs can be given orally, by injection, or by inhalation.  Oral administration is the most convenient method but inhalation therapy is the most effective treatment for relief of airway obstruction. As with anti-inflammatory therapy, administration of bronchodilators by inhalation requires the use of a special mask (see Figure 15 above).  Clinically useful bronchodilators drugs are outlined in the chart that follows:




(approved by FDA for use in horse, May '98)

b 2 adrenergic receptor agonist Oral Broncho-
dilation; Stimulation
of muco-
and mucus secretion
12 hours Tachy-
Pirbuterol (Maxair) b 2 adrenergic receptor agonist Aerosol Broncho-
dilation; Stimulation of muco-
escalator and mucus
1-2 hours Minimal since adminis-
tered by aerosol rather than systemically
Albuterol (Ventolin) b 2 adrenergic receptor agonist Aerosol Broncho-
dilation; Stimulation of muco-
escalator and mucus
1-2 hours Minimal since adminis-
tered by aerosol rather than systemically
Ephedrine Both a and b 2 receptors agonist Oral Broncho-
Efficacy and duration never tested in clinical trials Stimulation of central nervous system
Atropine Nonspecific muscarinic antagonist IV Broncho-
4-6 hours Gastrointestinal stasis and colic
Ipratropium bromide (Atrovent) Quaternary ammonium nonspecific muscarinic receptor antagonist Aerosol Broncho-
4-6 hours Minimal since poorly absorbed from airways into blood
line (a derivative of theophylline)
Nonspecific inhibition of cAMP and cGMP phosphodi-
Oral or IV Broncho-
Duration of effect never tested in clinical trials Excitation, nervousness, increased heart rate at doses required for bronchodilation

For information on the mechanism of action of bronchodilator drugs, click here.





This article was prepared by Rachele J. Baker under the direction of the faculty and staff of the Equine Pulmonary Laboratory.